See how Dr. Lee Polite makes chromatography simple
READ – WATCH – OR BOTH
Advance your skills with our HPLC & GC educational articles and videos
Chromatography has played a crucial role in advancing new discoveries across various scientific fields. This separation technique is fundamental to innovations in drug discovery, environmental analysis, food safety, and much more.
Before I talk about front tailing HPLC peaks, aka peak fronting, let’s differentiate peak fronting with peak tailing.
What is a peak tail? That is when the back half, or the later half of a peak tails. Check out my video HPLC peak tailing.
Peak tailing is one of the biggest problems in chromatography.
Unfortunately, peaks tail in HPLC. We don’t want them to tail. In theory we like to think of peaks as Gaussian shaped and perfectly symmetrical, but in reality just about everything in chromatography has a slight tail on it - about a 5% tail.
I’ll give you three quick tips for GC method development.
Hi there, it is Lee Polite again from Axion labs and today I am going to answer one of the toughest questions in the GC world. That is, “how do you develop a method for GC?”
Need help choosing the right HPLC column?
"How do I choose the right HPLC column?" It seems like a tough question and I hear it often. But don't worry! I've been teaching chromatography courses for over 30 years and I'd love to help you out.
There is a lot that goes into answering this question.
I usually give an hour-long lecture on the role of pH in HPLC during my hands-on HPLC courses.
So first let me give you a big picture view and then dive into the most important details.