HPLC & GC Education
See how Dr. Lee Polite makes chromatography simple
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Advance your skills with our HPLC & GC educational articles and videos
Chromatography has played a crucial role in advancing new discoveries across various scientific fields. This separation technique is fundamental to innovations in drug discovery, environmental analysis, food safety, and much more.
HPLC Peak Tailing
Peak tailing is one of the biggest problems in chromatography.
Unfortunately, peaks tail in HPLC. We don’t want them to tail. In theory we like to think of peaks as Gaussian shaped and perfectly symmetrical, but in reality just about everything in chromatography has a slight tail on it - about a 5% tail.
GC Method Development Training (Video) – Top 3 Tips
I’ll give you three quick tips for GC method development.
Hi there, it is Lee Polite again from Axion labs and today I am going to answer one of the toughest questions in the GC world. That is, “how do you develop a method for GC?”
How To Choose An HPLC Column (Video)
Need help choosing the right HPLC column?
"How do I choose the right HPLC column?" It seems like a tough question and I hear it often. But don't worry! I've been teaching chromatography courses for over 30 years and I'd love to help you out.
HPLC Mobile Phases: Acetonitrile vs Methanol – Which One Is Better?
Two of the most popular HPLC mobile phases go head to head. Which one is the winner for you depends on a few key considerations.
Cannabis – Using HPLC and GC To Measure Cannabinoids
This is a new industry - for Axion Labs - and that is exciting from an analytical chemistry perspective.
The cannabis labs popping up all over the country have a couple of challenges.
How to Select a Buffer for your HPLC Mobile Phase?
There is a lot that goes into answering this question.
I usually give an hour-long lecture on the role of pH in HPLC during my hands-on HPLC courses.
So first let me give you a big picture view and then dive into the most important details.
Choose a Chromatography Learning Path
Take the drivers seat of your HPLC/GC and your career.