HPLC & GC Education
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Chromatography has played a crucial role in advancing new discoveries across various scientific fields. This separation technique is fundamental to innovations in drug discovery, environmental analysis, food safety, and much more.
Monomeric vs Polymeric C18 Columns
Let's dive into a slightly more obscure topic today: monomeric versus polymeric C18s.
When to use an HPLC gradient?
The big question is, how do you know when you should use a gradient elution instead of isocratic elution?
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Can I run an HPLC gradient from 0 to 100%?
Can I run my instrument from 0 to 100% or do I have to stop at 5 to 95%?
Dwell Volume vs Dead Volume, What’s The Difference?
Dwell volume and dead volume can effect your chromatography. What's the difference between the two?
How to calculate void volume in HPLC?
In the realm of High Performance Liquid Chromatography (HPLC), the precise measurement of T0, or the time at which solutes elute without interaction with the stationary phase, is fundamental.
Can You Use 100% Water with a C18 column?
Have you ever wondered if you can run your C18 column with 100% water? Here's the straightforward answer: We don't recommend it? Why?
HPLC problems with very polar molecules
Very polar molecules will cause problems if the correct HPLC column is not being used.There's a specific type of HPLC column known as the polar embedded phase.
Understanding HPLC Guard Columns: Necessity and Best Practices
Guard columns play a critical role in chromatography, acting as a protective barrier for the more expensive analytical columns.
UPLC and HPLC, What’s the difference? (Video)
Let me give you the technical difference. UPLC, first of all, is a registered trademark of Waters Corporation. Other companies call there's something like UHPLC or STM or something other than UPLC.
How to Clean a C18 Column?
If you have the suspicion that your C18 column isn’t working as well as it did, you may be wondering if cleaning it will help. I’ll give you a couple easy things to do that cleans the column well.
HPLC Tubing & Fittings
Here's another set of questions that involves the tubing and fittings that we use in the LC. So, this is like the nuts and bolts—literally the nuts and bolts in the LC system.
Fixing the #1 Most Common Problem in HPLC: An Air Bubble in the Pump
If you want to develop technical mastery in any domain, you need to know how to quickly solve common problems. So let me show you how to fix the most common problem in HPLC.
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