HPLC & GC Education

See how Dr. Lee Polite makes chromatography simple

READ  –  WATCH  –  OR BOTH

Advance your skills with our HPLC & GC educational articles and videos

Chromatography has played a crucial role in advancing new discoveries across various scientific fields. This separation technique is fundamental to innovations in drug discovery, environmental analysis, food safety, and much more.

HPLC Flow Rates: Everything You Need to Know 

HPLC Flow Rates: Everything You Need to Know 

Understanding the relationship between column diameter and HPLC flow rate. The question often arises: "What's the HPLC flow rate? What's it based on?" The answer is simpler than it seems.
How to Test an LC Column 

How to Test an LC Column 

You cleaned a LC column now you say "Hey Lee, I've got this column. It was looking really garbagey, and I think I fixed it. How do I know it's good? Is there a way to test a LC column?"
Best Solvent To Dissolve HPLC Samples

Best Solvent To Dissolve HPLC Samples

In this article we delve into the critical aspect of selecting the perfect sample solvent for effective chromatography. The choice of solvent for dissolving your sample is crucial because it directly affects the chromatography process.
Dwell Volume vs Dead Volume, What’s The Difference?

Dwell Volume vs Dead Volume, What’s The Difference?

Dwell volume and dead volume can effect your chromatography. What's the difference between the two?
How to calculate void volume in HPLC?

How to calculate void volume in HPLC?

In the realm of High Performance Liquid Chromatography (HPLC), the precise measurement of T0, or the time at which solutes elute without interaction with the stationary phase, is fundamental.
Can You Use 100% Water with a C18 column?

Can You Use 100% Water with a C18 column?

Have you ever wondered if you can run your C18 column with 100% water? Here's the straightforward answer: We don't recommend it? Why?
HPLC problems with very polar molecules

HPLC problems with very polar molecules

Very polar molecules will cause problems if the correct HPLC column is not being used.There's a specific type of HPLC column known as the polar embedded phase.
Understanding HPLC Guard Columns: Necessity and Best Practices

Understanding HPLC Guard Columns: Necessity and Best Practices

Guard columns play a critical role in chromatography, acting as a protective barrier for the more expensive analytical columns.
UPLC and HPLC, What’s the difference? (Video)

UPLC and HPLC, What’s the difference? (Video)

Let me give you the technical difference. UPLC, first of all, is a registered trademark of Waters Corporation. Other companies call there's something like UHPLC or STM or something other than UPLC.

Choose a Chromatography Learning Path

Take the drivers seat of your HPLC/GC and your career.