Virtual LC/GC Bootcamp

18 Fun Lectures | 10 Fun Labs

This is the recorded version of our in-person and hands-on LC/GC Bootcamp. We recorded this course at Axion Labs & Training Institute on Oct. 18-22, 2021. The foundation for the virtual course is the recorded lectures.

Axion has provided advanced chromatography training to every major pharmaceutical, chemical, and petroleum company in the US since 1999.

There are millions of HPLCs and GCs in labs at companies like these around the world.
They are always looking for top professionals with valuable skills.
And they want their employees to develop technical mastery.

The LC/GC Virtual Course Will Give You Advanced Skills

Your ultimate online course to level-up your chromatography skills from wherever you are.

This is the recorded version of our in-person and hands-on LC/GC Bootcamp.  The foundation for the virtual course is the recorded lectures.

Lectures: You will get the recorded lectures plus course slides that teach you the science behind chromatography

Labs: Dr. Lee Polite will walk you through each lab experiment. 

GC: In the 18 lectures and 10 labs you will learn

Choose the right inlet for your application. Split/splitless, multi-mode inlets, cool on-column, etc. 

See how oven temperature impacts the separation. 

Master the resolution equation

Maximize efficiency by understanding the VanDeemter and Golay equations.

How to choose and install a column.

What detector to use? TCD, FID..

9-step funnel that will enable you to troubleshoot your GC.

And more.

Dr. Polite and an HPLC

HPLC/UPLC: In the 8 lectures and 3 labs you will learn

5 different modes of HPLC: reversed phase, ion pair, normal phase, ion exchange, and size exclusion.

Install a column.

Adjust parameters to control capacity, selectivity and efficiency.

UV-Vis detectors. Refractive index (RI) detectors.

When to choose reversed phase column.

Troubleshoot common problems. Pressure fluctuations, baseline fluctuations, ghost peaks, tailing peaks, fronting peaks, doubled peaks.

And more.

By the end of this course you will be able to take a complete unknown for the GC and the HPLC and you will develop new methods using everything that you have learned.

You will deliver a method with adequate separation and then optimize that method for resolution, speed and sensitivity.

Success Means More Than Just Pushing Buttons.

Master how LCs and GCs work and the science behind separation.

You have a AS, BS, MS or even a PhD.
You can follow standard procedures.
Can you choose the right stationary phase, particle size, flow rate, column dimensions, mobile phases, and buffers and create a robust optimized HPLC method?
Can you select the correct column chemistry, length, column diameter, film thickness, carrier gas, and temperature gradient to develop a GC method?
Can you install and change columns and perform maintenance on both HPLCs and GCs?
Can you efficiently troubleshoot and solve issues with separation and operations of your HPLCs and GCs?

Course Curriculum

Day 1

Introduction to GC

GC Inlets

Lab 1: Introduction to GC + Temperature


Lab 2: Split Ratio 

Lab 3: Split/Splitless

Day 2

GC Theory 

Capillary Columns

Lab 4: GC Parameters

Lab 5: GC Quant

Qualitative and Quantitative Analysis

Lab 6: Column Installation and Maintenance

Day 3

GC Detectors

GC/MS Talk and Demo 

Introduction to HPLC

Lab 7: LC Parameters

HPLC Instrumentation 

Resolution and Theory Review

Day 4

HPLC Detectors 

HPLC Columns   

Reversed Phase of Acids and Bases

Lab 8: Determination of Caffeine in Soft Drinks

HPLC Troubleshooting

LC Method Development

Lab 9: LC Method Development

Day 5

 GC Method Development

Lab 10: GC Method Development 

GC Troubleshooting

This course is fast paced and fun!  Yes, hard to believe but not only do we learn chromatography we also have fun while doing it. 


What Our Students Say

"Axion HPLC training course with Dr. Polite was a great, non-intimidating experience! I went in knowing little about chromatography, but Dr. Polite taught in a way that was easy to understand, using analogies and knowing the information inside and out. Not only did this course broaden my knowledge, it helped me land my first job using chromatography! This course is for anyone who wants to increase his or her marketability and become thoroughly educated in chromatography."
Savannah Hahn
Chicago, Illinois
"Dr. Polite's courses are incredible, they are very hands on with instrumentation and I strongly believe that regardless of your level of knowledge with GC or HPLC, 99% of you will walk away with something you can apply to your own studies/experiments/methods etc. If your work will pay for it or if you have any interest at all, GO TAKE THE CLASS!"
Lindsay Truman
Chicago, Illinois

"Hi I just wanted to take a minute to tell you what an amazing course Axion’s LC/GC Bootcamp is. I was fortunate enough to attend Lee’s course in the summer of 2021.  I’m a trained chromatographer with a focus in mass spectrometry.  Despite having a PhD in the field with years of study behind me, I was able to garner new information to expand my knowledge base. I highly encourage anyone to attend his course if you are afforded the opportunity."

Tiffany Liden, Ph.D.
Postdoc Researcher, Adjunct Professor  Department of Chemistry and Biochemistry, The University of Texas at Arlington
"Over the course of my career, I have attended several GC and HPLC trainings. This was the first training in which I left the course with a level of understanding that I could maximize the equipment. Rather than leaving with a tip or a trick for method development, Dr. Polite provided a complete roadmap to method development from theory to application in the training. It was enjoyable to go from the art of method development to the science of method development. For anyone looking to learn GC/HPLC, increase their lab skill sets, or make the transition from experienced user to expert; I would highly recommend this course."
Joe Block
"I'm glad I had the opportunity to attend two of the courses Axion offers. Dr. Lee Polite is one of the best teachers I ever met and his knowledge over the LC subject is amazing. I definitely recommend anyone that wants to learn about chromatography to attend the courses offered by this institute."
Klleber Oliveira
Senior Scientist, Quality Control USANA Health Sciences Inc.
"The team at Axion Labs offers a level of personalized and high-quality instruction that is nearly impossible to find anywhere else. Given how challenging of a technique chromatography is, I was blown away by the ability of every instructor to break it down into lessons that were easy to understand and retain. I came into an Axion class knowing the bare minimum about chromatography and left with the tools/confidence needed to solve various chromatography problems in the lab."
Stephen Braunewell
Associate Scientist II bluebird bio
"...was a game changer for my career. It single handedly helped me get every job I have ever had.  I was in metrology at APP pharmaceuticals. It helped me move to AD at a startup and later a third party field service tech and now owning my own service business. I am very thankful for my experience."
Michele McClain
Owner LakeShore Scientific
"Clearly explained topic with excellent detail, gave examples of applications relevant to my job application."
Cathleen Roush
Air Pollution Specialist Air Resource Board, California
"Great Instructor and staff! I enjoyed the "hands-on" part of this course. This course really broke down GC and the fundamentals."
Carla Reguen
Forensic Chemist Wilmington Police Dept. Crime Lab
"This course was extremely helpful in teaching the fundamentals of GC. The instructor presented the material in a fashion that was easily understandable."
Monika Kothari
Lab Technician Nalco Water

The LC/GC Virtual Bootcamp


Receive immediate access to the course.

Bonus: After buy the course, if you choose to come to an in-person LC/GC Bootcamp you will get a discount of the price you payed for the course.