Virtual LC/GC Bootcamp

18 Lectures
10 Labs

This is the recorded version of our in-person and hands-on LC/GC Bootcamp. We recorded this course at Axion Labs & Training Institute on Oct. 18-22, 2021. The foundation for the virtual course is the recorded lectures.



This is the online HPLC course and online GC course that everyone has been asking about.

You get the video lectures from the LC/GC Bootcamp with lifetime updates and a one year membership to the “Axion Labs Chromatography Cohort”. 

You will be given access to the Virtual LC/GC Bootcamp recorded lectures and labs.

Your ultimate guide to level-up your chromatography skills from wherever you are.

This course covers a wide variety of topics, from learning the fundamental principles of chromatographic separation to troubleshooting and method development – for both HPLC and GC.  This course will give you the confidence and skills to solve your separation problems and become a valued contributor in your lab, company or organization.  Check out the sample schedule below.  This is the exact schedule we follow in our $3995 in-person LC/GC Bootcamp.  You will have access to the recorded lectures, slides and labs.


A new community for HPLC and GC practitioners and experts.

We haven’t fully defined what this community will look like (we want to hear what you want), but here are a few ideas that we are considering:

Tutorials for the top software packages used for HPLC and GC.

Monthly Zoom meetings for problem solving.

Exclusive video content reviewing new HPLC and GC products.

Chat support for urgent troubleshooting.

An on-line community to share ideas, network and geek out about HPLC, GC, chemistry, science, etc.


Day 1 :

*All times are in CST
*Lab’s will not be live streamed

9:00 Introduction to GC

10:00 GC Inlets

11:00 Lab 1: Introduction to GC + Temperature

12:00 Lunch provided by Axion

1:00 Resolution

2:00 Lab 2: Split Ratio 

4:00 Lab 3: Split/Splitless

Day 2 :

*All times are in CST
*Lab’s will not be live streamed

9:00 GC Theory 

9:45 Capillary Columns

10:45 Lab 4: GC Parameters

11:45 Lunch provided by Axion

12:45 Lab 5: GC Quant

2:30 Qualitative and Quantitative Analysis

3:30 Lab 6: Column Installation and Maintenance

7:00 Dinner provided by Axion


Day 3 :

*All times are in CST
*Lab’s will not be live streamed

9:00 GC Detectors 

10:00 GC/MS Talk and Demo 

11:00 Introduction to HPLC

11:45 Lunch provided by Axion

1:45 Lab 7: LC Parameters

3:30 HPLC Instrumentation 

4:30 Resolution and Theory Review 

Day 4 :

*All times are in CST
*Lab’s will not be live streamed

9:00 HPLC Detectors 

10:00 HPLC Columns   

11:00 Reversed Phase of Acids and Bases

12:00 Lunch provided by Axion

1:00 Lab 8: Determination of Caffeine in Soft Drinks

2:00 HPLC Troubleshooting

3:00 LC Method Development

4:00 Lab 9: LC Method Development

Day 5 :

*All times are in CST
*Lab’s will not be live streamed

9:00 GC Method Development 

10:30 Lab 10: GC Method Development 

12:30 Lunch provided by Axion

1:30 GC Troubleshooting

3:00 End of Course

Dr. Lee Polite


Dr. Polite earned his Ph.D. in Chemistry (Chromatography) from Virginia Tech where he worked under the direction of Professor Harold McNair. Dr. McNair is recognized as one of the premier chromatography professors in the world and one of the pioneers in the field.


What Our Students Say


Hi I just wanted to take a minute to tell you what an amazing course Axion’s LC/GC Bootcamp is. I was fortunate enough to attend Lee’s course in the summer of 2021.  I’m a trained chromatographer with a focus in mass spectrometry.  Despite having a PhD in the field with years of study behind me, I was able to garner new information to expand my knowledge base. I highly encourage anyone to attend his course if you are afforded the opportunity.

Tiffany Liden, Ph.D.
Postdoc Researcher, Adjunct Professor  Department of Chemistry and Biochemistry, The University of Texas at Arlington
{Over the course of my career, I have attended several GC and HPLC trainings. This was the first training in which I left the course with a level of understanding that I could maximize the equipment. Rather than leaving with a tip or a trick for method development, Dr. Polite provided a complete roadmap to method development from theory to application in the training. It was enjoyable to go from the art of method development to the science of method development. For anyone looking to learn GC/HPLC, increase their lab skill sets, or make the transition from experienced user to expert; I would highly recommend this course.
Joe Block
{I'm glad I had the opportunity to attend two of the courses Axion offers. Dr. Lee Polite is one of the best teachers I ever met and his knowledge over the LC subject is amazing. I definitely recommend anyone that wants to learn about chromatography to attend the courses offered by this institute.
Klleber Oliveira
Senior Scientist, Quality Control USANA Health Sciences Inc.
{The team at Axion Labs offers a level of personalized and high-quality instruction that is nearly impossible to find anywhere else. Given how challenging of a technique chromatography is, I was blown away by the ability of every instructor to break it down into lessons that were easy to understand and retain. I came into an Axion class knowing the bare minimum about chromatography and left with the tools/confidence needed to solve various chromatography problems in the lab.  
Stephen Braunewell
Associate Scientist II bluebird bio
{...was a game changer for my career. It single handedly helped me get every job I have ever had.  I was in metrology at APP pharmaceuticals. It helped me move to AD at a startup and later a third party field service tech and now owning my own service business. I am very thankful for my experience.
Michele McClain
Owner LakeShore Scientific
{Clearly explained topic with excellent detail, gave examples of applications relevant to my job application.
Cathleen Roush
Air Pollution Specialist Air Resource Board, California
{Great Instructor and staff! I enjoyed the "hands-on" part of this course. This course really broke down GC and the fundamentals.
Carla Reguen
Forensic Chemist Wilmington Police Dept. Crime Lab
{This course was extremely helpful in teaching the fundamentals of GC. The instructor presented the material in a fashion that was easily understandable.
Monika Kothari
Lab Technician Nalco Water