Virtual LC/GC Bootcamp

18 Fun Lectures | 10 Fun Labs

This is the recorded version of our in-person and hands-on LC/GC Bootcamp. We recorded this course at Axion Labs & Training Institute on Oct. 18-22, 2021. The foundation for the virtual course is the recorded lectures.


This is the online HPLC course and online GC course that everyone has been asking about.

You get the video lectures from the LC/GC Bootcamp with lifetime updates.

You will be given access to the Virtual LC/GC Bootcamp recorded labs.

Your ultimate guide to level-up your chromatography skills from wherever you are.

This course covers a wide variety of topics, from learning the fundamental principles of chromatographic separation to troubleshooting and method development – for both HPLC and GC.  This course will give you the confidence and skills to solve your separation problems and become a valued contributor in your lab, company or organization.

Check out the course curriculum below.  This is the exact schedule we follow in our $4,495.00 in-person LC/GC Bootcamp.  You will have access to the recorded lectures, slides and labs.


Course Curriculum

Day 1

Introduction to GC

GC Inlets

Lab 1: Introduction to GC + Temperature


Lab 2: Split Ratio 

Lab 3: Split/Splitless

Day 2

GC Theory 

Capillary Columns

Lab 4: GC Parameters

Lab 5: GC Quant

Qualitative and Quantitative Analysis

Lab 6: Column Installation and Maintenance

Day 3

GC Detectors

GC/MS Talk and Demo 

Introduction to HPLC

Lab 7: LC Parameters

HPLC Instrumentation 

Resolution and Theory Review

Day 4

HPLC Detectors 

HPLC Columns   

Reversed Phase of Acids and Bases

Lab 8: Determination of Caffeine in Soft Drinks

HPLC Troubleshooting

LC Method Development

Lab 9: LC Method Development

Day 5

 GC Method Development

Lab 10: GC Method Development 

GC Troubleshooting

This course is fast paced and fun!  Yes, hard to believe but not only do we learn chromatography we also have fun while doing it. 


What Our Students Say


Hi I just wanted to take a minute to tell you what an amazing course Axion’s LC/GC Bootcamp is. I was fortunate enough to attend Lee’s course in the summer of 2021.  I’m a trained chromatographer with a focus in mass spectrometry.  Despite having a PhD in the field with years of study behind me, I was able to garner new information to expand my knowledge base. I highly encourage anyone to attend his course if you are afforded the opportunity.

Tiffany Liden, Ph.D.
Postdoc Researcher, Adjunct Professor  Department of Chemistry and Biochemistry, The University of Texas at Arlington
{Over the course of my career, I have attended several GC and HPLC trainings. This was the first training in which I left the course with a level of understanding that I could maximize the equipment. Rather than leaving with a tip or a trick for method development, Dr. Polite provided a complete roadmap to method development from theory to application in the training. It was enjoyable to go from the art of method development to the science of method development. For anyone looking to learn GC/HPLC, increase their lab skill sets, or make the transition from experienced user to expert; I would highly recommend this course.
Joe Block
{I'm glad I had the opportunity to attend two of the courses Axion offers. Dr. Lee Polite is one of the best teachers I ever met and his knowledge over the LC subject is amazing. I definitely recommend anyone that wants to learn about chromatography to attend the courses offered by this institute.
Klleber Oliveira
Senior Scientist, Quality Control USANA Health Sciences Inc.
{The team at Axion Labs offers a level of personalized and high-quality instruction that is nearly impossible to find anywhere else. Given how challenging of a technique chromatography is, I was blown away by the ability of every instructor to break it down into lessons that were easy to understand and retain. I came into an Axion class knowing the bare minimum about chromatography and left with the tools/confidence needed to solve various chromatography problems in the lab.  
Stephen Braunewell
Associate Scientist II bluebird bio
{...was a game changer for my career. It single handedly helped me get every job I have ever had.  I was in metrology at APP pharmaceuticals. It helped me move to AD at a startup and later a third party field service tech and now owning my own service business. I am very thankful for my experience.
Michele McClain
Owner LakeShore Scientific
{Clearly explained topic with excellent detail, gave examples of applications relevant to my job application.
Cathleen Roush
Air Pollution Specialist Air Resource Board, California
{Great Instructor and staff! I enjoyed the "hands-on" part of this course. This course really broke down GC and the fundamentals.
Carla Reguen
Forensic Chemist Wilmington Police Dept. Crime Lab
{This course was extremely helpful in teaching the fundamentals of GC. The instructor presented the material in a fashion that was easily understandable.
Monika Kothari
Lab Technician Nalco Water