Advanced Topics In GC

4-Day Course with 12 Hands-on Lab Experiments

I promise that you will leave this course with the knowledge to take on any chromatography challenge.

This class picks up where the popular “GC Fundamentals” and “LC/GC Bootcamp” leave off.  Those are not “beginner” classes by any means.  They take anyone from beginner to expert in one week.  Once you have a firm grasp of those GC fundamentals, you are ready for the advanced GC course which offers more breadth of GC topics…and 12 opportunities to get some hands-on experience with the new topics. 

You’ll get to use metal clad columns to run high temperature GC to investigate adulterated olive oils.

You’ll physically convert a GC from helium to hydrogen and see the analysis times get cut in half.

You will learn how to improve your sensitive 2500 fold by injecting 25 uL directly into the GC. 

You’ll get to do all of this while having fun…the Axion way!

Course Curriculum

Day 1

9:00 Introduction

9:15 Master Resolution Equation

10:15 Lab – Temperature and Flow Effects 

11:15 Injection Techniques (Part 1)

12:00 Lunch (provided by Axion)

1:00 Injection Techniques (Part 2)

2:00 Lab – S/SL/OC/Pulsed

3:30 Lab – Gas Sampling Valve 

4:00 GC Theory

Day 2

9:00 Method Development Examples

10:30 Lab – High Temperature

11:30 Stationary Phase Selection

12:00 Lunch (provided by Axion)

1:00 GC/MS

2:00 Lab – GC/MS

3:00 Derivatization in GC

4:00 Lab – Derivatization of Benzodiazepines

Day 3

9:00 Headspace

10:00 SPME

10:30 Lab – Quant of Alcohols by Headspace GC

12:00 Lunch (provided by Axion)

1:00 Lab – Method Development – Selectivity

2:00 Lab – Method Development Cut the Column

3:00 Lab – Helium vs Hydrogen for Carrier Gas

4:00 End of Course

Day 4

9:00 Troubleshooting GC Problems

10:30 Lab – Injector Maintenance

11:30 Lab – Detector Maintenance

1:00 End of Course

*Note that this is just a guide for the course.  The actual agenda will be determined based on interest of the participants.

Have you taken the LC/GC Bootcamp?

The LC/GC Bootcamp is the best chromatography training to start with for most people.

Take the LC/GC Bootcamp first, then either (or both) of the Advanced Topics courses. If you follow that learning path, we promise you will gain technical mastery in both HPLC and GC. You will feel confident knowing you can solve almost any HPLC or GC challenge you face.


4-Day Advanced GC Course

Hands-On Experience With New Topics 

Where: Axion Labs and Training Institute, Chicago Illinois

Instuctor: Dr. Lee N. Polite

July 16 – 19, 2024

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What Our Students Say

"Axion HPLC training course with Dr. Polite was a great, non-intimidating experience! I went in knowing little about chromatography, but Dr. Polite taught in a way that was easy to understand, using analogies and knowing the information inside and out. Not only did this course broaden my knowledge, it helped me land my first job using chromatography! This course is for anyone who wants to increase his or her marketability and become thoroughly educated in chromatography."
Savannah Hahn
Chicago, Illinois
"Dr. Polite's courses are incredible, they are very hands on with instrumentation and I strongly believe that regardless of your level of knowledge with GC or HPLC, 99% of you will walk away with something you can apply to your own studies/experiments/methods etc. If your work will pay for it or if you have any interest at all, GO TAKE THE CLASS!"
Lindsay Truman
Chicago, Illinois

"Hi I just wanted to take a minute to tell you what an amazing course Axion’s LC/GC Bootcamp is. I was fortunate enough to attend Lee’s course in the summer of 2021.  I’m a trained chromatographer with a focus in mass spectrometry.  Despite having a PhD in the field with years of study behind me, I was able to garner new information to expand my knowledge base. I highly encourage anyone to attend his course if you are afforded the opportunity."

Tiffany Liden, Ph.D.
Postdoc Researcher, Adjunct Professor  Department of Chemistry and Biochemistry, The University of Texas at Arlington
"Over the course of my career, I have attended several GC and HPLC trainings. This was the first training in which I left the course with a level of understanding that I could maximize the equipment. Rather than leaving with a tip or a trick for method development, Dr. Polite provided a complete roadmap to method development from theory to application in the training. It was enjoyable to go from the art of method development to the science of method development. For anyone looking to learn GC/HPLC, increase their lab skill sets, or make the transition from experienced user to expert; I would highly recommend this course."
Joe Block
"I'm glad I had the opportunity to attend two of the courses Axion offers. Dr. Lee Polite is one of the best teachers I ever met and his knowledge over the LC subject is amazing. I definitely recommend anyone that wants to learn about chromatography to attend the courses offered by this institute."
Klleber Oliveira
Senior Scientist, Quality Control USANA Health Sciences Inc.
"The team at Axion Labs offers a level of personalized and high-quality instruction that is nearly impossible to find anywhere else. Given how challenging of a technique chromatography is, I was blown away by the ability of every instructor to break it down into lessons that were easy to understand and retain. I came into an Axion class knowing the bare minimum about chromatography and left with the tools/confidence needed to solve various chromatography problems in the lab."
Stephen Braunewell
Associate Scientist II bluebird bio
"...was a game changer for my career. It single handedly helped me get every job I have ever had.  I was in metrology at APP pharmaceuticals. It helped me move to AD at a startup and later a third party field service tech and now owning my own service business. I am very thankful for my experience."
Michele McClain
Owner LakeShore Scientific
"Clearly explained topic with excellent detail, gave examples of applications relevant to my job application."
Cathleen Roush
Air Pollution Specialist Air Resource Board, California
"Great Instructor and staff! I enjoyed the "hands-on" part of this course. This course really broke down GC and the fundamentals."
Carla Reguen
Forensic Chemist Wilmington Police Dept. Crime Lab
"This course was extremely helpful in teaching the fundamentals of GC. The instructor presented the material in a fashion that was easily understandable."
Monika Kothari
Lab Technician Nalco Water

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