Advanced Topics in HPLC

4-Day Course with 10 Hands-on Lab Experiments

I promise you will leave this class with the knowledge to take on any chromatography challange.

This HPLC training course has a prerequisite.  You must have taken either the LC/GC Bootcamp or a previous course at Axion Labs.  Once you have a firm grasp of those HPLC fundamentals, you are ready for the advanced HPLC course which offers more breadth of LC topics…and 10 opportunities to get some hands-on experience with the new topics.  You’ll get a chance to 

  • Utilize HILIC to measure sugar content in adulterated fruit juices
  • Develop a method to simultaneously measure caffeine, artificial sweeteners and preservatives in soft drinks;
  • Convert HPLC methods to UPLC …and cut the analysis time in half!
  • Identify unknowns with the help from the 3-D capabilities of the Diode Array Dectctor
  • Analyze Hemp samples to determine cannabinoid concentrations
  • Tear apart and rebuild a modern HPLC Pump

And you’ll get to do all of this while having fun…the Axion way!

Course Curriculum

Day 1

9:00 Review of HPLC Resolution and Theory

10:45 Lab 1 – Advanced HPLC Parameters 

12 Lunch (provided by Axion)

1 PM Lab Review

1:30 Column Hardware

2:30 Lab 2 – Column Dimensions 

4:00 Lab Review

4:15 Solid Phase Micro Extraction (SPME)

5 PM End of Day

Day 2

9:00 Reversed Phase Mechanism and pH Effects (and HILIC?)

10:15 Lab 3 – pH Effects – Run pH and Caffeine standards at pH of 5.5, 4.7 and 4.0 

11:30 Quantitative Analysis

12 Lunch (provided by Axion)

1 PM Method Development and Gradients

2:00 Lab 4:  Qual/Quant of Soft Drinks

3:30 Lab Review

3:45 Lab 5 – HILIC HPLC of Sugars

5 PM End of Day

Day 3

9:00 Ion Chromatography – Dionex Sugars

9:30 Lab 6 – Dionex Sugars 

10:00 Sample Preparation

11:00 Lab 7 Amino Acids by Pre-Column Derivatization

12 Noon Lunch (provided by Axion)

1 PM Lab 8 – Method Optimization 

2:30 Troubleshooting

4:00 End of Day

Day 4

9:00 Troubleshooting HPLC

10:30 Lab 9 – Pump Maintenance 

11:30 Lab 10 – Detector and Injector Maintenance

1:00 End of Course

*Extra Discussion Topics and Demonstrations:  LC/MS, SPE Separation of Kool Aid, DAD

Have you taken the LC/GC Bootcamp?

The LC/GC Bootcamp is the best chromatography training to start with for most people.

Take the LC/GC Bootcamp first, then either (or both) of the Advanced Topics courses. If you follow that learning path, we promise you will gain technical mastery in both HPLC and GC. You will feel confident knowing you can solve almost any HPLC or GC challenge you face.


4-Day Advanced HPLC Course

Hands-On Experience With New Topics

Where: Axion Labs and Training Institute, Chicago Illinois

Instuctor: Dr. Lee N. Polite

Date: Nov 12-15 2024

Request information

Sign up Here


What Our Students Say

"I'm glad I had the opportunity to attend two of the courses Axion offers. Dr. Lee Polite is one of the best teachers I ever met and his knowledge over the LC subject is amazing. I definitely recommend anyone that wants to learn about chromatography to attend the courses offered by this institute."
Klleber Oliveira
Senior Scientist, Quality Control USANA Health Sciences Inc.

Choose a Chromatography Learning Path

Take control of your HPLC, GC and career.