Cleaning a reverse-phase HPLC column is easier than you might think. With just two steps—water first, then organic solvent—you can flush out salts, buffers, and sticky residues that drag down your separations. Follow this recipe, and your column often feels like new....
Most chromatographers know whether they’re using a C18 or a C8 column. You most likely know their particle size. But very few stop to consider two finer details that can make a big difference in your separations: HPLC carbon load and surface area. These are the...
Let’s do a deep dive into HPLC particles. Specifically, the difference between totally porous and superficially porous materials—and why it matters for efficiency and pressure. Quick Answer: SPS vs TPS in HPLC Totally porous HPLC particles provide large surface area...
Chromatography is the most widely used analytical tool in modern chemistry. Whether it’s gas chromatography (GC) or high-performance liquid chromatography (HPLC), nearly every chemical analysis in the world today depends on one of these techniques. But there’s one...
For any chromatographer, whether seasoned or just starting, the ultimate goal is to achieve good separation between analytes. This separation, known as resolution (Rs), is not a matter of chance but can be understood and manipulated through a fundamental relationship...
