by Lee Polite | Jan 12, 2026
HPLC Reproducibility Problems? It’s Not Your Method. If you are injecting the same sample and getting different chromatograms, you do not have a method problem. You have a reproducibility problem, and reproducibility issues almost always point to the instrument, not...
by Lee Polite | Nov 25, 2025
Most chromatographers have a love–hate relationship with their HPLC columns. We love them when they work beautifully, separating the toughest mixtures with clean, sharp peaks. But we hate the fear—the fear of ruining a $1,000 column with a single wrong step. So here’s...
by Lee Polite | Nov 18, 2025
When troubleshooting an HPLC method, one of the most common questions we hear is: “What column should I use when C18 doesn’t seem to work?” A recent webinar, What if C18 Doesn’t work?, question from an attendee captured this perfectly: “How does water interact...
by Lee Polite | Sep 10, 2025
Cleaning a reverse-phase HPLC column is easier than you might think. With just two steps—water first, then organic solvent—you can flush out salts, buffers, and sticky residues that drag down your separations. Follow this recipe, and your column often feels like new....
by Lee Polite | Sep 3, 2025
Most chromatographers know whether they’re using a C18 or a C8 column. You most likely know their particle size. But very few stop to consider two finer details that can make a big difference in your separations: HPLC carbon load and surface area. These are the...
