HPLC Flow Rates: Everything You Need to Know 

HPLC Flow Rates: Everything You Need to Know 

Understanding the relationship between column diameter and flow rate. The question often arises: “What’s the HPLC flow rate? What’s it based on?” The answer is simpler than it seems. I’ll explain how to pinpoint the perfect flow rate the...
How to Test an LC Column 

How to Test an LC Column 

I showed you how to clean a LC column in a different blog. Now you say: “Hey Lee, I’ve got this column. It was looking really garbagey, and I think I fixed it. How do I know it’s good? Is there a way to test a LC column?” Using Probes to Test...
Best Solvent To Dissolve HPLC Samples

Best Solvent To Dissolve HPLC Samples

In this article we delve into the critical aspect of selecting the perfect sample solvent for effective chromatography. The choice of solvent for dissolving your HPLC sample is crucial because it directly affects the chromatography process. Impact of Sample Solvent on...
Monomeric vs Polymeric C18 Columns

Monomeric vs Polymeric C18 Columns

Let’s dive into a slightly more obscure topic today: monomeric versus polymeric C18s. Now, I know it sounds technical, but stick with me. We’re delving into the fine details of columns here. Exploring the Complex World of C18 Columns In the realm of...
When to use an HPLC gradient?

When to use an HPLC gradient?

The big question is, how do you know when you should use a gradient elution instead of isocratic elution? To me, the way I always put it is, you’ll know if you’re waiting forever for the last peak to come off, then you probably need an HPLC gradient....