Hands-On HPLC & GC Training Courses

Master Chromatography

Solve Your Separation Problems

Advance Your Career

Gain Advanced HPLC & GC Skills.
Over 13,000 Professionals Trained.

Upcoming Events 

LC/GC Bootcamp July 29-Aug 2

The course everyone should start with.

    LC/GC Bootcamp Sep 16-20

    The course everyone should start with.

      LC/GC Bootcamp – Oct 14-18

      The course everyone should start with.

        The HPLC & GC training course everyone should start with.

        Rookie to Pro? Experienced to Expert? After completing this course you will have advanced HPLC and GC skills no matter your starting point.

        LC/GC Bootcamp

        Learning two applications of separation science at the same time reinforces and deepens your understanding of both techniques.

        The Axion Course Line Up

        After taking our hands-on HPLC & GC training courses, you will know more about HPLC and GC than 95% of the professionals who do GC or HPLC for a living! That is a bold statement to make, but it’s true. Axion has been providing the finest professional chromatography training to every major pharmaceutical, chemical, and petroleum company in the US for more than 30 years.

        Virtual Course

        Can’t travel to Chicago?  This is the on-demand virtual HPLC & GC course that everyone has been asking about. You will be given access to the Virtual LC/GC Bootcamp recorded lectures and labs.

        Hands-on LC/GC Bootcamp

        You will learn HPLC and GC theory, operation, troubleshooting, maintenance and method development and optimization.  You can expect to spend much of the week in the lab practicing what you learn.

        Hands-on Advanced GC

        Once you have a firm grasp of GC fundamentals, you are ready for the advanced GC course which offers more breadth of GC topics…and 12 advanced lab experiments to get some hands-on experience with the new topics.

        The LC/GC Bootcamp is prerequisite.

        Hands-on Advanced HPLC

        Once you have a firm grasp of HPLC fundamentals, you are ready for the advanced HPLC course which offers more breadth of LC topics…and 10 opportunities to get some hands-on experience with the new topics.

        The LC/GC Bootcamp is prerequisite.

        Check Out Our Educational Videos And Articles

        Let us know your needs and we promise to help you find the best path.

        We can train 1 or 100.  Submit your info so we can contact you and provide your organization with a training solution and skills that you need.

        I will help you master HPLC & GC.

        You know that chromatography is an essential skill.  Our HPLC and GC courses will help you develop a competitive and valuable skill set.

        We have been teaching chromatography for 30 years and I promise – you CAN learn chromatography.

        What Our Students Say

        "Dr. Polite's courses are incredible, they are very hands on with instrumentation and I strongly believe that regardless of your level of knowledge with GC or HPLC, 99% of you will walk away with something you can apply to your own studies/experiments/methods etc. If your work will pay for it or if you have any interest at all, GO TAKE THE CLASS!"
        Lindsay Truman
        Chicago, Illinois

        "Hi I just wanted to take a minute to tell you what an amazing course Axion’s LC/GC Bootcamp is. I was fortunate enough to attend Lee’s course in the summer of 2021.  I’m a trained chromatographer with a focus in mass spectrometry.  Despite having a PhD in the field with years of study behind me, I was able to garner new information to expand my knowledge base. I highly encourage anyone to attend his course if you are afforded the opportunity."

        Tiffany Liden, Ph.D.
        Postdoc Researcher, Adjunct Professor  Department of Chemistry and Biochemistry, The University of Texas at Arlington
        "Over the course of my career, I have attended several GC and HPLC trainings. This was the first training in which I left the course with a level of understanding that I could maximize the equipment. Rather than leaving with a tip or a trick for method development, Dr. Polite provided a complete roadmap to method development from theory to application in the training. It was enjoyable to go from the art of method development to the science of method development. For anyone looking to learn GC/HPLC, increase their lab skill sets, or make the transition from experienced user to expert; I would highly recommend this course."
        Joe Block
        "I'm glad I had the opportunity to attend two of the courses Axion offers. Dr. Lee Polite is one of the best teachers I ever met and his knowledge over the LC subject is amazing. I definitely recommend anyone that wants to learn about chromatography to attend the courses offered by this institute."
        Klleber Oliveira
        Senior Scientist, Quality Control USANA Health Sciences Inc.
        "The team at Axion Labs offers a level of personalized and high-quality instruction that is nearly impossible to find anywhere else. Given how challenging of a technique chromatography is, I was blown away by the ability of every instructor to break it down into lessons that were easy to understand and retain. I came into an Axion class knowing the bare minimum about chromatography and left with the tools/confidence needed to solve various chromatography problems in the lab."
        Stephen Braunewell
        Associate Scientist II bluebird bio
        "...was a game changer for my career. It single handedly helped me get every job I have ever had.  I was in metrology at APP pharmaceuticals. It helped me move to AD at a startup and later a third party field service tech and now owning my own service business. I am very thankful for my experience."
        Michele McClain
        Owner LakeShore Scientific
        "Clearly explained topic with excellent detail, gave examples of applications relevant to my job application."
        Cathleen Roush
        Air Pollution Specialist Air Resource Board, California
        "Great Instructor and staff! I enjoyed the "hands-on" part of this course. This course really broke down GC and the fundamentals."
        Carla Reguen
        Forensic Chemist Wilmington Police Dept. Crime Lab
        "This course was extremely helpful in teaching the fundamentals of GC. The instructor presented the material in a fashion that was easily understandable."
        Monika Kothari
        Lab Technician Nalco Water

        The Axion difference



        Dr. Lee Polite has been teaching chromatography to professionals for over 30 years. He has been invited to lecture on the topic in 17 countries and has trained over 12,000 professionals.


        As the gold standard for chromatography training, Axion has provided hands-on training to every major pharmaceutical, chemical, and petroleum company in the US, along with all leading government labs.


        Through an immersive experience in the heart of downtown Chicago, Axion will take you from beginner to expert in both GC and LC, in just 5 days!

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