LC/GC Bootcamp

Five-Day Hands-On HPLC & GC Training Course
Over 12,000 Trained Professionals

18 Fun Lectures | 10 Hands-On Labs

Learning HPLC and GC at the same time? “This is the way.”

The fundamental concept to understand GC and HPLC is separation.  So when you learn HPLC and GC you learn two applications of separation science.  This reinforces and deepens your understanding.

So in our opinion – based on training over 12,000 professionals – learning HPLC helps you understand GC better and vice versa.

Even if you currently use only one of these techniques, you will gain valuable skills that increase your marketability.

See what our students say.

What You Can Expect To Learn
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HPLC & GC Theory & Fundamentals
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Operation of modern HPLC's and GC's
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HPLC & GC Maintenance
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HPLC & GC Troubleshooting
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HPLC & GC Method Development
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Mass spectrometry fundamentals
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Quantitative Analysis

Who qualifies to take this course?

No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course.

All students need is a desire to learn HPLC and GC.

Axion has been providing  chromatography training to every major pharmaceutical, chemical, and petroleum company in the US for more than 25 years.

This course is designed for users of ANY familiarity level who want practical laboratory experience and thorough understanding of the technique fundamentals.

By the end of this course, students will feel comfortable operating any modern GC or HPLC.  All of the classroom instruction is reinforced with plenty of hands-on time with HPLCs and GCs.

See what our students say.

This course is ideal for:
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Aspiring professionals in analytical chemistry.
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Rising managers or mid-level analysts who want to add depth and breadth to their technical mastery.
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Medicinal chemists
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Your employees who use HPLC and GC
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BS/MS level chemists who have little hands-on experience
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Anyone who has a desire to learn about HPLC and GC

Course Curriculum

Day 1

9:00 Introduction to GC

10:00 GC Inlets

11:00 Lab 1: Introduction to GC + Temperature

12:00 Lunch provided by Axion

1:00 Resolution

2:00 Lab 2: Split Ratio 

4:00 Lab 3: Split/Splitless

Day 2

9:00 GC Theory 

9:45 Capillary Columns

10:45 Lab 4: GC Parameters

11:45 Lunch provided by Axion

12:45 Lab 5: GC Quant

2:30 Qualitative and Quantitative Analysis

3:30 Lab 6: Column Installation and Maintenance

7:00 Dinner provided by Axion

Day 3

9:00 GC Detectors

10:00 GC/MS Talk and Demo 

11:00 Introduction to HPLC

11:45 Lunch provided by Axion

1:45 Lab 7: LC Parameters

3:30 HPLC Instrumentation 

4:30 Resolution and Theory Review

Day 4

9:00 HPLC Detectors 

10:00 HPLC Columns   

11:00 Reversed Phase of Acids and Bases

12:00 Lunch provided by Axion

1:00 Lab 8: Determination of Caffeine in Soft Drinks

2:00 HPLC Troubleshooting

3:00 LC Method Development

4:00 Lab 9: LC Method Development

Day 5

9:00 GC Method Development

10:30 Lab 10: GC Method Development 

12:30 Lunch provided by Axion

1:30 GC Troubleshooting

3:00 End of Course

This course is fast paced and fun!  Yes, hard to believe but not only do we learn chromatography we also have fun while doing it.  Axion takes students out to lunch daily and also plans evening activities around Chicago.

Registration

5-Day LC/GC Bootcamp Course

Learn HPLC and GC at the same time.

Where: Axion Labs and Training Institute, Chicago Illinois

Instuctor: Dr. Lee N. Polite

Choose Your Date

Sep 26-30, 2022

Mar 13-17, 2023

Jun 12-16, 2023

Aug 21-25, 2023

Oct 16-20, 2023

Dec 12-16, 2022

May 1-5, 2023

Jul 17-21, 2023

Sep 11-15, 2023

Dec 4-8, 2023

Meet The Instructor

Experienced Chromatographer  |  Experienced Instuctor

Dr. Lee N. Polite

Importance of Innovation in Gas Chromatography

Lee Polite is the president and founder of Axion Analytical Labs, Inc. Since its inception in 1995, Axion has provided in-depth chromatography training to every major pharmaceutical, chemical, and petroleum company in the US. Lee has been teaching chromatography to professionals for more than 30 years. He has been invited to lecture on the topic in 17 countries and has trained more than 12000 professionals. Axion’s Chicago training facility is equipped with 8 HPLCs and 16 GCs all dedicated to Axion’s hands-on training courses.

Dr. Polite earned his Ph.D. in Chemistry (Chromatography) from Virginia Polytechnic Institute and State University (Virginia Tech) where he worked under the direction of Professor Harold McNair. Dr. McNair is recognized as one of the premier chromatography professors in the world and one of the pioneers in the field.

What Our Students Say

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Hi I just wanted to take a minute to tell you what an amazing course Axion’s LC/GC Bootcamp is. I was fortunate enough to attend Lee’s course in the summer of 2021.  I’m a trained chromatographer with a focus in mass spectrometry.  Despite having a PhD in the field with years of study behind me, I was able to garner new information to expand my knowledge base. I highly encourage anyone to attend his course if you are afforded the opportunity.

Tiffany Liden, Ph.D.
Postdoc Researcher, Adjunct Professor  Department of Chemistry and Biochemistry, The University of Texas at Arlington
{Over the course of my career, I have attended several GC and HPLC trainings. This was the first training in which I left the course with a level of understanding that I could maximize the equipment. Rather than leaving with a tip or a trick for method development, Dr. Polite provided a complete roadmap to method development from theory to application in the training. It was enjoyable to go from the art of method development to the science of method development. For anyone looking to learn GC/HPLC, increase their lab skill sets, or make the transition from experienced user to expert; I would highly recommend this course.
Joe Block
{I'm glad I had the opportunity to attend two of the courses Axion offers. Dr. Lee Polite is one of the best teachers I ever met and his knowledge over the LC subject is amazing. I definitely recommend anyone that wants to learn about chromatography to attend the courses offered by this institute.
Klleber Oliveira
Senior Scientist, Quality Control USANA Health Sciences Inc.
{The team at Axion Labs offers a level of personalized and high-quality instruction that is nearly impossible to find anywhere else. Given how challenging of a technique chromatography is, I was blown away by the ability of every instructor to break it down into lessons that were easy to understand and retain. I came into an Axion class knowing the bare minimum about chromatography and left with the tools/confidence needed to solve various chromatography problems in the lab.  
Stephen Braunewell
Associate Scientist II bluebird bio
{...was a game changer for my career. It single handedly helped me get every job I have ever had.  I was in metrology at APP pharmaceuticals. It helped me move to AD at a startup and later a third party field service tech and now owning my own service business. I am very thankful for my experience.
Michele McClain
Owner LakeShore Scientific
{Clearly explained topic with excellent detail, gave examples of applications relevant to my job application.
Cathleen Roush
Air Pollution Specialist Air Resource Board, California
{Great Instructor and staff! I enjoyed the "hands-on" part of this course. This course really broke down GC and the fundamentals.
Carla Reguen
Forensic Chemist Wilmington Police Dept. Crime Lab
{This course was extremely helpful in teaching the fundamentals of GC. The instructor presented the material in a fashion that was easily understandable.
Monika Kothari
Lab Technician Nalco Water

Chromatography Learning Paths

Take the drivers seat of your HPLC/GC and of your career.