LC/GC Bootcamp
Five-Day Hands-On HPLC & GC Training Course
Over 13,000 Trained Professionals
18 Fun Lectures | 10 Hands-On Labs
Learning HPLC and GC at the same time? “This is the way.”
The fundamental concept to understand GC and HPLC is separation. So when you learn HPLC and GC you learn two applications of separation science. This reinforces and deepens your understanding.
So in our opinion – based on training over 12,000 professionals – learning HPLC helps you understand GC better and vice versa.
Even if you currently use only one of these techniques, you will gain valuable skills that increase your marketability.
What You Can Expect To Learn
HPLC & GC Theory & Fundamentals
Operation of modern HPLC's and GC's
HPLC & GC Maintenance
HPLC & GC Troubleshooting
HPLC & GC Method Development
Mass spectrometry fundamentals
Quantitative Analysis
Who qualifies to take this course?
No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course.
All students need is a desire to learn HPLC and GC.
Axion has been providing chromatography training to every major pharmaceutical, chemical, and petroleum company in the US for more than 25 years.
This course is designed for users of ANY familiarity level who want practical laboratory experience and thorough understanding of the technique fundamentals.
By the end of this course, students will feel comfortable operating any modern GC or HPLC. All of the classroom instruction is reinforced with plenty of hands-on time with HPLCs and GCs.
This course is ideal for:
Aspiring professionals in analytical chemistry.
Rising managers or mid-level analysts who want to add depth and breadth to their technical mastery.
Medicinal chemists
Your employees who use HPLC and GC
BS/MS level chemists who have little hands-on experience
Anyone who has a desire to learn about HPLC and GC
Course Curriculum
Day 1
9:00 Introduction to GC
10:00 GC Inlets
11:00 Lab 1: Introduction to GC + Temperature
12:00 Lunch provided by Axion
1:00 Resolution
2:00 Lab 2: Split Ratio
4:00 Lab 3: Split/Splitless
Day 2
9:00 GC Theory
9:45 Capillary Columns
10:45 Lab 4: GC Parameters
11:45 Lunch provided by Axion
12:45 Lab 5: GC Quant
2:30 Qualitative and Quantitative Analysis
3:30 Lab 6: Column Installation and Maintenance
7:00 Dinner provided by Axion
Day 3
9:00 GC Detectors
10:00 GC/MS Talk and Demo
11:00 Introduction to HPLC
11:45 Lunch provided by Axion
1:45 Lab 7: LC Parameters
3:30 HPLC Instrumentation
4:30 Resolution and Theory Review
Day 4
9:00 HPLC Detectors
10:00 HPLC Columns
11:00 Reversed Phase of Acids and Bases
12:00 Lunch provided by Axion
1:00 Lab 8: Determination of Caffeine in Soft Drinks
2:00 HPLC Troubleshooting
3:00 LC Method Development
4:00 Lab 9: LC Method Development
Day 5
9:00 GC Method Development
10:30 Lab 10: GC Method Development
12:30 Lunch provided by Axion
1:30 GC Troubleshooting
3:00 End of Course
This course is fast paced and fun! Yes, hard to believe but not only do we learn chromatography we also have fun while doing it. Axion takes students out to lunch daily and also plans evening activities around Chicago.
Registration
5-Day LC/GC Bootcamp Course
Learn HPLC and GC at the same time.
Where: Axion Labs and Training Institute, Chicago Illinois
Instuctor: Dr. Lee N. Polite
Choose Your Date
$3,995.00
Mar 13-17, 2023
Jun 12-16, 2023
Aug 21-25, 2023
Oct 16-20, 2023
May 1-5, 2023
Jul 17-21, 2023
Sep 11-15, 2023
Dec 4-8, 2023
*If you are a legal professional reach out to us here.
Meet The Instructor
Experienced Chromatographer | Experienced Instuctor
Dr. Lee N. Polite

Lee Polite is the president and founder of Axion Analytical Labs, Inc. Since its inception in 1995, Axion has provided in-depth chromatography training to every major pharmaceutical, chemical, and petroleum company in the US. Lee has been teaching chromatography to professionals for more than 30 years. He has been invited to lecture on the topic in 17 countries and has trained more than 12000 professionals. Axion’s Chicago training facility is equipped with 8 HPLCs and 16 GCs all dedicated to Axion’s hands-on training courses.
Dr. Polite earned his Ph.D. in Chemistry (Chromatography) from Virginia Polytechnic Institute and State University (Virginia Tech) where he worked under the direction of Professor Harold McNair. Dr. McNair is recognized as one of the premier chromatography professors in the world and one of the pioneers in the field.
What Our Students Say
Chromatography Learning Paths
Take the drivers seat of your HPLC/GC and of your career.