LC/GC Bootcamp



After this course, every student knows more about LC and GC chromatography than 80% of the professionals who do GC or HPLC for a living! That is a bold statement to make, but it’s true. Axion has been providing the finest professional chromatography training to every major pharmaceutical, chemical, and petroleum company in the US for more than 25 years. These companies are repeat customers because the participants love our classes and their employers love the value provided by well-trained and more productive employees.

This course is designed for users of ANY familiarity level who want practical laboratory experience and thorough understanding of the technique fundamentals. By the end of this course, students will feel comfortable operating any modern GC/LC. As an added bonus, the instructor makes it both simple and fun to learn. All of the classroom instruction is reinforced with plenty of hands-on time with HPLCs and GCs.

Every participant will have the opportunity to build a calibration curve, develop complex methods from scratch, and optimize GC methods and LC Methods to separate complex samples in less than 1 minute! No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course. All students need is a desire to learn HPLC and GC.

This course is fast paced and fun! Yes,  hard to believe but not only do we learn chromatography we also have fun while doing it.  Axion takes students out to lunch daily and also plan activities around Chicago at night.

Day 1 :

9:00 Introduction to GC

10:00 GC Inlets

11:00 Resolution

12:00 Lunch provided by Axion

1:00 Lab 1: Introduction to GC + Temperature

2:00 Lab 2: Split Ratio 

4:00 Lab 3: Split/Splitless + MMI Demo

Day 2 :

9:00 GC Theory 

9:45 Capillary Columns

10:45 Lab 4: GC Parameters

11:45 Lunch provided by Axion

12:45 Lab 5: Column Installation and Maintenance

2:30 Qualitative and Quantitative Analysis

3:30 Lab 6: GC Quant

7:00 Dinner provided by Axion


Day 3 :

9:00 GC/MS Talk and Demo 

10:00 Introduction to HPLC

10:45 HPLC Instrumentation

11:45 Lunch provided by Axion

12:45 Resolution and Theory Review

1:45 Lab 7: LC Parameters

3:00 HPLC Detectors

4:00 HPLC Columns

Day 4 :

9:00 Reversed Phase of Acids and Bases

10:00 Lab 8: Determination of Caffeine in Soft Drinks  

11:00 HPLC Troubleshooting

12:00 Lunch provided by Axion

1:00 LC Method Development

2:30 Lab 9: LC Method Development

4:00 GC Headspace

Day 5 :

9:00 Lab 10: GC Headspace Quant

10:30 GC Method Development

11:30 GC Troubleshooting

12:30 Lunch provided by Axion

1:30 Lab 11: GC Method Development

3:00 End of Course

Dr. Lee Polite


Dr. Polite earned his Ph.D. in Chemistry (Chromatography) from Virginia Tech where he worked under the direction of Professor Harold McNair. Dr. McNair is recognized as one of the premier chromatography professors in the world and one of the pioneers in the field.


What Our Students Say

{Over the course of my career, I have attended several GC and HPLC trainings. This was the first training in which I left the course with a level of understanding that I could maximize the equipment. Rather than leaving with a tip or a trick for method development, Dr. Polite provided a complete roadmap to method development from theory to application in the training. It was enjoyable to go from the art of method development to the science of method development. For anyone looking to learn GC/HPLC, increase their lab skill sets, or make the transition from experienced user to expert; I would highly recommend this course.
Joe Block
{I'm glad I had the opportunity to attend two of the courses Axion offers. Dr. Lee Polite is one of the best teachers I ever met and his knowledge over the LC subject is amazing. I definitely recommend anyone that wants to learn about chromatography to attend the courses offered by this institute.
Klleber Oliveira
Senior Scientist, Quality Control USANA Health Sciences Inc.
{The team at Axion Labs offers a level of personalized and high-quality instruction that is nearly impossible to find anywhere else. Given how challenging of a technique chromatography is, I was blown away by the ability of every instructor to break it down into lessons that were easy to understand and retain. I came into an Axion class knowing the bare minimum about chromatography and left with the tools/confidence needed to solve various chromatography problems in the lab.  
Stephen Braunewell
Associate Scientist II bluebird bio
{...was a game changer for my career. It single handedly helped me get every job I have ever had.  I was in metrology at APP pharmaceuticals. It helped me move to AD at a startup and later a third party field service tech and now owning my own service business. I am very thankful for my experience.
Michele McClain
Owner LakeShore Scientific
{Clearly explained topic with excellent detail, gave examples of applications relevant to my job application.
Cathleen Roush
Air Pollution Specialist Air Resource Board, California
{Great Instructor and staff! I enjoyed the "hands-on" part of this course. This course really broke down GC and the fundamentals.
Carla Reguen
Forensic Chemist Wilmington Police Dept. Crime Lab
{This course was extremely helpful in teaching the fundamentals of GC. The instructor presented the material in a fashion that was easily understandable.
Monika Kothari
Lab Technician Nalco Water

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