LC/GC Bootcamp



After this course, every student knows more about LC and GC chromatography than 80% of the professionals who do GC or HPLC for a living! That is a bold statement to make, but it’s true. Axion has been providing the finest professional chromatography training to every major pharmaceutical, chemical, and petroleum company in the US for more than 25 years. These companies are repeat customers because the participants love our classes and their employers love the value provided by well-trained and more productive employees.

This course is designed for users of ANY familiarity level who want practical laboratory experience and thorough understanding of the technique fundamentals. By the end of this course, students will feel comfortable operating any modern GC/LC. As an added bonus, the instructor makes it both simple and fun to learn. All of the classroom instruction is reinforced with plenty of hands-on time with HPLCs and GCs.

Every participant will have the opportunity to build a calibration curve, develop complex methods from scratch, and optimize GC methods and LC Methods to separate complex samples in less than 1 minute! No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course. All students need is a desire to learn HPLC and GC.

This course is fast paced and fun! Yes,  hard to believe but not only do we learn chromatography we also have fun while doing it.  Axion takes students out to lunch daily and also plan activities around Chicago at night.

Day 1 :

9:00 Introduction to GC

10:00 GC Inlets

11:00 Resolution

12:00 Lunch provided by Axion

1:00 Lab 1: Introduction to GC + Temperature

2:00 Lab 2: Split Ratio 

4:00 Lab 3: Split/Splitless + MMI Demo

Day 2 :

9:00 GC Theory 

9:45 Capillary Columns

10:45 Lab 4: GC Parameters

11:45 Lunch provided by Axion

12:45 Lab 5: Column Installation and Maintenance

2:30 Qualitative and Quantitative Analysis

3:30 Lab 6: GC Quant

7:00 Dinner provided by Axion


Day 3 :

9:00 GC/MS Talk and Demo 

10:00 Introduction to HPLC

10:45 HPLC Instrumentation

11:45 Lunch provided by Axion

12:45 Resolution and Theory Review

1:45 Lab 7: LC Parameters

3:00 HPLC Detectors

4:00 HPLC Columns

Day 4 :

9:00 Reversed Phase of Acids and Bases

10:00 Lab 8: Determination of Caffeine in Soft Drinks  

11:00 HPLC Troubleshooting

12:00 Lunch provided by Axion

1:00 LC Method Development

2:30 Lab 9: LC Method Development

4:00 GC Headspace

Day 5 :

9:00 Lab 10: GC Headspace Quant

10:30 GC Method Development

11:30 GC Troubleshooting

12:30 Lunch provided by Axion

1:30 Lab 11: GC Method Development

3:00 End of Course

Dr. Lee Polite


Dr. Polite earned his Ph.D. in Chemistry (Chromatography) from Virginia Tech where he worked under the direction of Professor Harold McNair. Dr. McNair is recognized as one of the premier chromatography professors in the world and one of the pioneers in the field.

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