HPLC & GC Education
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Advance your skills with our HPLC & GC educational articles and videos
Chromatography has played a crucial role in advancing new discoveries across various scientific fields. This separation technique is fundamental to innovations in drug discovery, environmental analysis, food safety, and much more.
UPLC and HPLC, What’s the difference? (Video)
Let me give you the technical difference. UPLC, first of all, is a registered trademark of Waters Corporation. Other companies call there's something like UHPLC or STM or something other than UPLC.
How to Clean a C18 Column?
If you have the suspicion that your C18 column isn’t working as well as it did, you may be wondering if cleaning it will help. I’ll give you a couple easy things to do that cleans the column well.
HPLC Tubing & Fittings
Here's another set of questions that involves the tubing and fittings that we use in the LC. So, this is like the nuts and bolts—literally the nuts and bolts in the LC system.
Fixing the #1 Most Common Problem in HPLC: An Air Bubble in the Pump
If you want to develop technical mastery in any domain, you need to know how to quickly solve common problems. So let me show you how to fix the most common problem in HPLC.
HPLC Sample Prep Basics
Let's talk about sample prep, because this is where most of the errors occur in the entire analytical procedure.
HPLC – Negative Peaks and Baseline Drift
We had two cool questions come in recently, and I can solve both with one simple answer. We're going to talk about negative peaks and baseline drift in HPLC.
Ghost Peaks in HPLC – 5 common sources
Ghost peaks means there are peaks that showed up that were not expected. There is something there, so we have to figure out where that something is coming from.
How long does it take a column to equilibrate?
A lot of people ask the question, "How long does it take a column to equilibrate?"
There is an exact answer. You don't need to guess.
Problems Caused By Dwell Volume
Dwell volume is a critical concept in chromatography that can significantly impact the accuracy and reproducibility of your analytical results.
What is dwell volume? The basics
Trying to understand the concept of dwell volume in HPLC? Dwell volume is defined as the volume between where your gradient is formed to where it touches the column.
HPLC Basics Video: Analyzing a New Sample
Learning principles of analytical chemistry and HPLC will help you analyze your own samples from scratch.
Agilent? Waters? Shimadzu? Does it matter which one I use?
Agilent? Waters? Shimadzu? Does it matter which one I use?
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