HPLC & GC Education
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Chromatography has played a crucial role in advancing new discoveries across various scientific fields. This separation technique is fundamental to innovations in drug discovery, environmental analysis, food safety, and much more.
In gas chromatography what is column flow, purge flow, split ratio?
Column Flow, Purge Flow, and Split Ratio all relate to the movement and control of gas flow within a gas chromatograph (GC) injector system.
They are essential for sample introduction and separation efficiency.
What is the Difference Between Running Green Stainless vs. Red Stainless HPLC Tubing at 0.3 mL/min?
Flow rates between 1.0 and 2.0 mL/min, green tubing is optimal. However, when operating at very low flow rates—such as 0.3 mL/min—green tubing is too large.
Can acetonitrile cause amine impurities to form deposits in HPLC pump?
I've read that acetonitrile can have amine impurities and form deposits in pump components over time. Is there a procedure for flushing the system to remove these components?
Chromatography Flow Rates Explained: HPLC, LC-MS & GC
When starting a new method, what is a good flow rate to begin with?
We will discuss HPLC, LC-MS & GC flow rates.
Chromatography Skills You Need to Get Hired and Promoted
The demand for skilled laboratory professionals continues to grow, and chromatography techniques remain dominant tools in nearly every industry.
HPLC Flow Rates: Everything You Need to Know
Understanding the relationship between column diameter and HPLC flow rate. The question often arises: "What's the HPLC flow rate? What's it based on?" The answer is simpler than it seems.
How to Test an LC Column
You cleaned a LC column now you say "Hey Lee, I've got this column. It was looking really garbagey, and I think I fixed it. How do I know it's good? Is there a way to test a LC column?"
Best Solvent To Dissolve HPLC Samples
In this article we delve into the critical aspect of selecting the perfect sample solvent for effective chromatography.
The choice of solvent for dissolving your sample is crucial because it directly affects the chromatography process.
Monomeric vs Polymeric C18 Columns
Let's dive into a slightly more obscure topic today: monomeric versus polymeric C18s.
When to use an HPLC gradient?
The big question is, how do you know when you should use a gradient elution instead of isocratic elution?
To ...
Can I run an HPLC gradient from 0 to 100%?
Can I run my instrument from 0 to 100% or do I have to stop at 5 to 95%?
Dwell Volume vs Dead Volume, What’s The Difference?
Dwell volume and dead volume can effect your chromatography. What's the difference between the two?
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