HPLC & GC Education
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Chromatography has played a crucial role in advancing new discoveries across various scientific fields. This separation technique is fundamental to innovations in drug discovery, environmental analysis, food safety, and much more.
GC Sample Prep Essentials: Nailing Solvents, Dilution & Injection
When you're working with Gas Chromatography (GC), one of the most critical steps is getting the sample into the GC. You might already understand gas flow paths and general instrument operation—but none of that matters if your sample isn’t prepared correctly.
Pyrolysis GC: How to Analyze Paint, Plastics, and Polymers with Gas Chromatography
Pyrolysis literally means “breaking down with heat.” In this technique, we take a very small solid sample, like a chip of plastic or paint, and burn it at high temperatures. As the material decomposes, it produces a mixture of gases—these are the pyrolysis products.
How to fix “GC-MS vial out of range” error?
Running into a "vial out of range" error on your Agilent GC or GCMS system? Don’t worry—it’s a common mistake with an easy fix. I'll explain what causes this error and how to solve it, especially if you're using an Agilent 7683 autosampler.
Reference wavelength, Peak Purity, and Threshold – Diode Array Parameters and Tools.
If the reference wavelength is activated, should the spectra range include the reference wavelength too? If not, what will happen?
I'm going open ChemStation to answer the question.
Will Highly Acidic or Basic Samples Affect an HPLC C18 Column?
The general rule in HPLC is that we always want to dissolve the sample in the mobile phase—that is the ideal situation. If the sample solvent is the same as the mobile phase, it will just disappear; it will blend in.
How to Test THC Potency – GC or HPLC?
In the cannabis world, the active ingredient is Delta-9 tetrahydrocannabinol (Delta-9 THC). Measuring potency typically means determining how much THC is in the sample. Do you use HPLC or GC?
In gas chromatography what is column flow, purge flow, split ratio?
Column Flow, Purge Flow, and Split Ratio all relate to the movement and control of gas flow within a gas chromatograph (GC) injector system.
They are essential for sample introduction and separation efficiency.
What is the Difference Between Running Green Stainless vs. Red Stainless HPLC Tubing at 0.3 mL/min?
Flow rates between 1.0 and 2.0 mL/min, green tubing is optimal. However, when operating at very low flow rates—such as 0.3 mL/min—green tubing is too large.
Can acetonitrile cause amine impurities to form deposits in HPLC pump?
I've read that acetonitrile can have amine impurities and form deposits in pump components over time. Is there a procedure for flushing the system to remove these components?
Chromatography Flow Rates Explained: HPLC, LC-MS & GC
When starting a new method, what is a good flow rate to begin with?
We will discuss HPLC, LC-MS & GC flow rates.
Chromatography Skills You Need to Get Hired and Promoted
The demand for skilled laboratory professionals continues to grow, and chromatography techniques remain dominant tools in nearly every industry.
HPLC Flow Rates: Everything You Need to Know
Understanding the relationship between column diameter and HPLC flow rate. The question often arises: "What's the HPLC flow rate? What's it based on?" The answer is simpler than it seems.
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