Chromatography is the most widely used analytical tool in modern chemistry. Whether it’s gas chromatography (GC) or high-performance liquid chromatography (HPLC), nearly every chemical analysis in the world today depends on one of these techniques. But there’s one...
For any chromatographer, whether seasoned or just starting, the ultimate goal is to achieve good separation between analytes. This separation, known as resolution (Rs), is not a matter of chance but can be understood and manipulated through a fundamental relationship...
RI detector showing negative peaks? It’s not a mistake! Most of us in the lab are pretty familiar with the sight of a good, strong positive peak on our chromatograms. It’s what we expect, right? But every now and then, especially if you’re working...
Understanding Reference Wavelength in Chromatography If the reference wavelength is activated, should the spectra range include the reference wavelength too? If not, what will happen? I’m going to walk over to my laptop and open ChemStation to answer the...
The General Rule in HPLC So this is in response to a video I did about the use of guard columns. The general rule in HPLC is that we always want to dissolve the sample in the mobile phase—that is the ideal situation. If the sample solvent is the same as the mobile...
