How to Clean a C18 Column?

How to Clean a C18 Column?

If you have the suspicion that your C18 column isn’t working as well as it did, you may be wondering if cleaning it will help. The good news is that you can clean a C18 column. I’ll give you a couple easy things to do that cleans the column well.  But first,...
HPLC Tubing & Fittings

HPLC Tubing & Fittings

Welcome back to our series on frequently asked questions in HPLC. Here’s another set of questions that involves the tubing and fittings that we use in the LC. Stainless Steel Tubing Vs PEEK Tubing Stainless Steel Fitting Vs PEEK Fitting Cautions About PEEK...
HPLC Sample Prep Basics

HPLC Sample Prep Basics

Let’s talk about HPLC sample prep, because this is where most of the errors occur in the entire analytical procedure. We call this pre-analytical error, and those are errors that happen before we get to the instrument. We could measure the precision, the...
HPLC – Negative Peaks and Baseline Drift

HPLC – Negative Peaks and Baseline Drift

We had two cool questions come in recently, and I can solve both with one simple answer. So, are you ready for this? We’re going to talk about negative peaks baseline drift in HPLC. How To Correct Negative Peaks And Baseline Drift? So both of those should be a...