In the realm of High Performance Liquid Chromatography (HPLC), the precise measurement of T0, or the time at which solutes elute without interaction with the stationary phase, is fundamental. Let’s discuss T0 and column volumes and explore measurement methods....
Have you ever wondered if you can run your C18 column with 100% water? Here’s the straightforward answer: We don’t recommend using 100% water with a C18 column. Why do we advise against it. The reason lies in the nature of the C18 itself. Known for its...
Very polar molecules will cause problems if the correct HPLC column is not being used. There’s a specific type of HPLC column for polar molecules. They are known as the polar embedded phase. Currently, every major column company has developed their version of...
Guard columns play a critical role in chromatography, acting as a protective barrier for the more expensive analytical columns. Essentially, guard columns are shorter versions of the analytical column, containing the same stationary phase and particle size....
Let me give you the technical difference between HPLC & UPLC. UPLC, first of all, is a registered trademark of Waters Corporation. Other companies call their version of UPLC something like UHPLC or STM or something other than UPLC. What is Ultra Performance Liquid...
