A lot of people ask the question, “How long does it take a column to equilibrate?” There is an exact answer. You don’t need to guess. It takes 5 column volumes to equilibrate a column. Now, you ask yourself the question, “What is a column...
Dwell volume is a critical concept in chromatography that can significantly impact the accuracy and reproducibility of your analytical results. What is Dwell Volume It refers to the volume between the pump and the column and plays a crucial role in the shape of the...
Trying to understand the concept of dwell volume in HPLC? Dwell volume is defined as the volume between where your gradient is formed to where it touches the column. It’s just a practical piece of plumbing. There’s a piece of tubing between the pump...
Watch the labinar with a free Axion Lite Membership. WATCH NOW Learning the basics of HPLC and some principle of analytical chemistry will help you confidently analyze your own samples from scratch. Have you ever wondered how much aspirin is in your aspirin tablet or...
You want chromatography training so you can solve your separation problems, advance your research and your career. We can help you achieve your goals, whether you’re looking for HPLC training or GC training. One question we often hear is, “What’s the difference...
