What is dwell volume?  The basics

What is dwell volume? The basics

Trying to understand the concept of dwell volume in HPLC?  Dwell volume is defined as the volume between where your gradient is formed to where it touches the column. It’s just a practical piece of plumbing. There’s a piece of tubing between the pump...
HPLC Basics Video: Analyzing a New Sample

HPLC Basics Video: Analyzing a New Sample

Watch the labinar with a free Axion Lite Membership. WATCH NOW Learning the basics of HPLC and some principle of analytical chemistry will help you confidently analyze your own samples from scratch. Have you ever wondered how much aspirin is in your aspirin tablet or...
LC/GC Bootcamp vs. Advanced Topics In HPLC & GC Courses

LC/GC Bootcamp vs. Advanced Topics In HPLC & GC Courses

You want chromatography training so you can solve your separation problems, advance your research and your career. We can help you achieve your goals, whether you’re looking for HPLC training or GC training. One question we often hear is, “What’s the difference...
What To Do When The HPLC Runs Dry

What To Do When The HPLC Runs Dry

Here’s a question that came in anonymously because they let their HPLC run dry and they are wondering what to do. So what do you do if your HPLC runs dry? How to prevent your HPLC from running dry First statement:  Don’t let it run dry. Here’s a trick: you can...
Front Tailing HPLC & GC Peaks

Front Tailing HPLC & GC Peaks

What are fronted tailing peaks in HPLC and GC?  Why do they occur in your chromatogram?  How can you reduce them and do better chromatography? Before I talk about front tailing HPLC peaks, aka peak fronting, let’s differentiate peak fronting with peak...