Guard columns play a critical role in chromatography, acting as a protective barrier for the more expensive analytical columns. Essentially, guard columns are shorter versions of the analytical column, containing the same stationary phase and particle size....
Let me give you the technical difference between HPLC & UPLC. UPLC, first of all, is a registered trademark of Waters Corporation. Other companies call their version of UPLC something like UHPLC or STM or something other than UPLC. What is Ultra Performance Liquid...
If you have the suspicion that your C18 column isn’t working as well as it did, you may be wondering if cleaning it will help. The good news is that you can clean a C18 column. I’ll give you a couple easy things to do that cleans the column well. But first,...
Welcome back to our series on frequently asked questions in HPLC. Here’s another set of questions that involves the tubing and fittings that we use in the LC. Stainless Steel Tubing Vs PEEK Tubing Stainless Steel Fitting Vs PEEK Fitting Cautions About PEEK...
If you want to develop technical mastery in any domain, you need to know how to quickly solve common problems. So let me show you how to fix the most common problem in HPLC: an air bubble in the pump. An air bubble in the pump is by far the number one cause of...
