The General Rule in HPLC So this is in response to a video I did about the use of guard columns. The general rule in HPLC is that we always want to dissolve the sample in the mobile phase—that is the ideal situation. If the sample solvent is the same as the mobile...
Understanding THC Potency Measurement In the cannabis world, the active ingredient is Delta-9 tetrahydrocannabinol (Delta-9 THC). Measuring potency typically means determining how much THC is in the sample. Chromatographic Separation of THC Chromatographically, THC...
The question “What is the difference between running green stainless vs. red stainless HPLC tubing at 0.3 mL/min?” arose from the video HPLC Tubing and Fittings. In this video I explored the differences between stainless steel and PEEK tubing, as well as the...
Does Acetonitrile Contain Amine Impurities That Affect HPLC Pumps? I’ve read that acetonitrile can have amine impurities and form deposits in pump components over time. Is there a procedure for flushing the system to remove these components? Is Acetonitrile Safe...
When starting a new method, what is a good flow rate to begin with? We will discuss chromatography flow rates for HPLC, LC-MS & GC. HPLC: Starting Flow Rates Let’s talk about HPLC. If you’re running LC, the typical flow rate on a standard diameter column is...
