RI detector showing negative peaks? It’s not a mistake! Most of us in the lab are pretty familiar with the sight of a good, strong positive peak on our chromatograms. It’s what we expect, right? But every now and then, especially if you’re working...
Understanding Reference Wavelength in Chromatography If the reference wavelength is activated, should the spectra range include the reference wavelength too? If not, what will happen? I’m going to walk over to my laptop and open ChemStation to answer the...
The General Rule in HPLC So this is in response to a video I did about the use of guard columns. The general rule in HPLC is that we always want to dissolve the sample in the mobile phase—that is the ideal situation. If the sample solvent is the same as the mobile...
Understanding THC Potency Measurement In the cannabis world, the active ingredient is Delta-9 tetrahydrocannabinol (Delta-9 THC). Measuring potency typically means determining how much THC is in the sample. Chromatographic Separation of THC Chromatographically, THC...
The question “What is the difference between running green stainless vs. red stainless HPLC tubing at 0.3 mL/min?” arose from the video HPLC Tubing and Fittings. In this video I explored the differences between stainless steel and PEEK tubing, as well as the...
