Does Acetonitrile Contain Amine Impurities That Affect HPLC Pumps? I’ve read that acetonitrile can have amine impurities and form deposits in pump components over time. Is there a procedure for flushing the system to remove these components? Is Acetonitrile Safe...
When starting a new method, what is a good flow rate to begin with? We will discuss chromatography flow rates for HPLC, LC-MS & GC. HPLC: Starting Flow Rates Let’s talk about HPLC. If you’re running LC, the typical flow rate on a standard diameter column is...
Understanding the relationship between column diameter and flow rate. The question often arises: “What’s the HPLC flow rate? What’s it based on?” The answer is simpler than it seems. I’ll explain how to pinpoint the perfect flow rate the...
I showed you how to clean a LC column in a different blog. Now you say: “Hey Lee, I’ve got this column. It was looking really garbagey, and I think I fixed it. How do I know it’s good? Is there a way to test a LC column?” Using Probes to Test...
In this article we delve into the critical aspect of selecting the perfect sample solvent for effective chromatography. The choice of solvent for dissolving your HPLC sample is crucial because it directly affects the chromatography process. Impact of Sample Solvent on...
