Advanced Topics In Chromatography
Master Complex HPLC & GC Techniques in Just 4 Days
Take Your Chromatography Skills to the Next Level
If you’ve already been to the LC/GC Bootcamp and want more, this course is for you.
The Advanced Topics in Chromatography course is a one-of-a-kind training program designed for chromatographers who want to optimize separations, troubleshoot complex issues and apply advanced analytical techniques with confidence.
Over four intensive days, you’ll work directly with cutting-edge instrumentation, gaining practical hands-on experience.
Take a deeper dive into:
method development & optimization
qualitative and quantitative analysis
mass spectrometry
HPLC & GC maintenance
This hands-on course, led by Dr. Lee Polite, merges the content of our Advanced HPLC and Advanced GC courses into a streamlined, expert-level program.
Why This Course?
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Covers BOTH HPLC & GC on an expert-level – Unlike traditional courses that focus on one technique, this course integrates both, offering a well-rounded, comparative approach to chromatography.
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Hands-On Learning – Work directly with instruments in real-world applications.
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Taught by Dr. Lee Polite – A leading chromatography expert with decades of experience training scientists worldwide.
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Tailored for Experienced Users – This course is designed for those who already understand the basics and want to develop expert-level skills. The LC/GC Bootcamp is a prerequisite.
Course Overview
Day 1: Fundamentals & Advanced HPLC Techniques
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Review of HPLC & GC theory and the master resolution equation
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Advanced HPLC parameters and method optimization
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Lab Exercises: pH effects on resolution, peak shape, and real-world applications like soft drink analysis
Day 2: GC Optimization & Injection Techniques
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Advanced GC method development, focusing on temperature and flow effects
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Split, splitless, and cold-on-column injection techniques
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High-temperature GC for challenging separations
Day 3: Mass Spectrometry & Headspace GC
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GC/MS best practices – Learn how to maximize sensitivity and accuracy
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Hands-on labs covering GC/MS, headspace analysis, and method optimization
Day 4: The Future of Chromatography
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Alternatives to helium in GC and method conversion from helium to hydrogen
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Preventative maintenance for both HPLC and GC systems
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Personalized Learning: Participants can choose a topic of interest for expert discussion and guidance
Who Should Attend?
This course is perfect for:
Graduates of the LC/GC Bootcamp
Analytical chemists and scientists looking to refine their chromatography expertise
Lab managers seeking method optimization strategies and troubleshooting skills
Researchers working with both HPLC & GC techniques in their studies
Have you taken the LC/GC Bootcamp?
The LC/GC Bootcamp is the best chromatography training to start with for most people.
Take the LC/GC Bootcamp first, then take a deep dive into chromatography with Advanced Topics in Chromatography.
If you follow that learning path, we promise you will gain technical mastery in both HPLC and GC. You will feel confident knowing you can solve almost any HPLC or GC challenge you face.
Advanced Topics Course Curriculum
Day 1
9:00 Review of HPLC and GC theory and master resolution equation
10:45 Lab 1 – Advanced HPLC parameters
12:00 Lunch
1:00 Lab 1 data review
1:30 Lab 2 – pH effects on resolution, peak shape etc. caffeine
3:00 Lab 2 data review
3:30 Lab 3 – Qualitative and quantitative analysis of soft drinks
5:00 Lab 3 data review
Day 2
9:00 Lab 4 – HPLC Method Optimization
10:30 Lab 4 data review
11:00 Lab 5 – Temperature and flow effects in GC resolution
12:00 Lunch
1:00 Lab 6 – Split, splitless and cold-on-culumn injection
3:30 Lab 7 – High temperature GC
4:30 Lab 7 review
Day 3
9:00 GC/MS theory and best practices
10:00 Lab 8 – GC/MS lab
11:30 Lab 8 review
12:00 lunch
1:00 Lab 9 – GC Headspace
2:30 Lab 10 – GC method development and optimization
5:00 lab 10 review
Day 4
9:00 Alternatives to helium for GC
9:30 Lab 11 – Method conversion from helium to hydrogen
10:30 Lab 11 data review
12:00 Lunch
1:00 HPLC maintenance
2:00 – GC maintenance
3:00 – Your selected topic
4:00 – Your selected topic
*Note that this is just a guide for the course. The actual agenda will be determined based on interest of the participants.
Registration
Spaces for this hands-on course are limited!
Get trained by a chromatography expert and master the techniques that set you apart in the lab.
4-Day Advanced Topics Course
Expert-level – Hands-On Experience
Where: Axion Labs and Training Institute, Chicago Illinois
Instuctor: Dr. Lee N. Polite
July 15-18, 2025
$4,995.00
Take Your Chromatography Skills to the Next Level
Master chromatography and take the drivers seat of your HPLC/GC and of your career.