What are fronted tailing peaks in HPLC and GC? Why do they occur in your chromatogram? How can you reduce them and do better chromatography? Before I talk about front tailing HPLC peaks, aka peak fronting, let’s differentiate peak fronting with peak...
Peak tailing is one of the biggest problems in chromatography. Unfortunately, peaks tail in HPLC. We don’t want them to tail. In theory we like to think of peaks as Gaussian shaped and perfectly symmetrical, but in reality just about everything in...
Register for my free, on-demand HPLC method development webinar. Need help choosing the right HPLC column? “How do I choose the right HPLC column?” It seems like a tough question and I hear it often. But don’t worry! I’ve been teaching...
Check out our free HPLC Method Development Webinar Choose the best HPLC mobile phase. Is it methanol or acetonitrile? Two of the most popular HPLC mobile phases go head to head. Which one is the winner for you depends on a few key considerations. Need more HPLC...
There is a lot that goes into answering this question. I usually give an hour-long lecture on the role of pH and buffers in HPLC mobile phases during my hands-on HPLC courses. So first let me give you a big picture view and then dive into the most...
