Let’s talk about HPLC sample prep, because this is where most of the errors occur in the entire analytical procedure. We call this pre-analytical error, and those are errors that happen before we get to the instrument. We could measure the precision, the...
We had two cool questions come in recently, and I can solve both with one simple answer. So, are you ready for this? We’re going to talk about negative peaks and baseline drift in HPLC. How To Correct Negative Peaks And Baseline Drift? So both of those should be...
A common problem happening in HPLC is what we call ghost peaks. Ghost peaks means there are peaks that showed up that were not expected. There is something there, so we have to figure out where that something is coming from. You have a sample, there are some peaks...
A lot of people ask the question, “How long does it take a column to equilibrate?” There is an exact answer. You don’t need to guess. It takes 5 column volumes to equilibrate a column. Now, you ask yourself the question, “What is a column...
Dwell volume is a critical concept in chromatography that can significantly impact the accuracy and reproducibility of your analytical results. What is Dwell Volume It refers to the volume between the pump and the column and plays a crucial role in the shape of the...
