Welcome back to our series on frequently asked questions in HPLC. Here’s another set of questions that involves the tubing and fittings that we use in the LC. Stainless Steel Tubing Vs PEEK Tubing Stainless Steel Fitting Vs PEEK Fitting Cautions About PEEK...
If you want to develop technical mastery in any domain, you need to know how to quickly solve common problems. So let me show you how to fix the most common problem in HPLC: an air bubble in the pump. An air bubble in the pump is by far the number one cause of...
Let’s talk about HPLC sample prep, because this is where most of the errors occur in the entire analytical procedure. We call this pre-analytical error, and those are errors that happen before we get to the instrument. We could measure the precision, the...
We had two cool questions come in recently, and I can solve both with one simple answer. So, are you ready for this? We’re going to talk about negative peaks and baseline drift in HPLC. How To Correct Negative Peaks And Baseline Drift? So both of those should be...
A common problem happening in HPLC is what we call ghost peaks. Ghost peaks means there are peaks that showed up that were not expected. There is something there, so we have to figure out where that something is coming from. You have a sample, there are some peaks...
