Advanced Topics in HPLC
4-Day Course with 10 Hands-on Lab Experiments
I promise you will leave this class with the knowledge to take on any chromatography challange.
The Advanced Topics in HPLC offers more breadth of LC topics…and 10 opportunities to get some hands-on experience with the new topics.
You’ll get a chance to
Utilize HILIC to measure sugar content in adulterated fruit juices
Develop a method to simultaneously measure caffeine, artificial sweeteners and preservatives in soft drinks.
Convert HPLC methods to UPLC …and cut the analysis time in half!
Identify unknowns with the help from the 3-D capabilities of the Diode Array Dectctor.
Analyze Hemp samples to determine cannabinoid concentrations.
Tear apart and rebuild a modern HPLC Pump.
This HPLC training course has a prerequisite.
You must have taken either the LC/GC Bootcamp or a previous course at Axion Labs. Once you have a firm grasp of those HPLC fundamentals, you are ready for the advanced HPLC course.
Course Curriculum
Day 1
9:00 Review of HPLC Resolution and Theory
10:45 Lab 1 – Advanced HPLC Parameters
12 Lunch (provided by Axion)
1 PM Lab Review
1:30 Column Hardware
2:30 Lab 2 – Column Dimensions
4:00 Lab Review
4:15 Solid Phase Micro Extraction (SPME)
5 PM End of Day
Day 2
9:00 Reversed Phase Mechanism and pH Effects (and HILIC?)
10:15 Lab 3 – pH Effects – Run pH and Caffeine standards at pH of 5.5, 4.7 and 4.0
11:30 Quantitative Analysis
12 Lunch (provided by Axion)
1 PM Method Development and Gradients
2:00 Lab 4: Qual/Quant of Soft Drinks
3:30 Lab Review
3:45 Lab 5 – HILIC HPLC of Sugars
5 PM End of Day
Day 3
9:00 Ion Chromatography – Dionex Sugars
9:30 Lab 6 – Dionex Sugars
10:00 Sample Preparation
11:00 Lab 7 Amino Acids by Pre-Column Derivatization
12 Noon Lunch (provided by Axion)
1 PM Lab 8 – Method Optimization
2:30 Troubleshooting
4:00 End of Day
Day 4
9:00 Troubleshooting HPLC
10:30 Lab 9 – Pump Maintenance
11:30 Lab 10 – Detector and Injector Maintenance
1:00 End of Course
*Extra Discussion Topics and Demonstrations: LC/MS, SPE Separation of Kool Aid, DAD
Registration
4-Day Advanced HPLC Course
Hands-On Experience With New Topics
Where: Axion Labs and Training Institute, Chicago Illinois
Instuctor: Dr. Lee N. Polite
2025 Dates TBD
$4,995.00
Have you taken the LC/GC Bootcamp?
The LC/GC Bootcamp is the best chromatography training to start with for most people.
Take the LC/GC Bootcamp first, then either (or both) of the Advanced Topics courses. If you follow that learning path, we promise you will gain technical mastery in both HPLC and GC. You will feel confident knowing you can solve almost any HPLC or GC challenge you face.