HPLC Training Course: AKA HPLC Bootcamp

Looking for an HPLC training course? HPLC only?  If you care less about gas chromatography, then this is the course for you!

You can start here on your learning path to master HPLC.  Interested in GC too?  Then start with our LC/GC Bootcamp!

HPLC Bootcamp


Spend four days with the HPLC training experts at Axion and know more about HPLC than 80% of the professionals who do HPLC for a living!

This sounds like marketing hype, but we know it’s true…and so do our thousands of satisfied customers! Axion has been providing the finest professional chromatography training to every major pharmaceutical, chemical, and petroleum company in the US for more than 25 years. These companies are repeat customers because the participants love our classes and their employers love the value provided by the well-trained and more productive employees.

This course is designed for HPLC users of ANY familiarity level who want practical laboratory experience and a thorough understanding of HPLC fundamentals. By the end of this course, students will feel comfortable operating any modern HPLC system and choosing the correct solvent strength, flow rate, particle size, column length, column diameter, and stationary phase. HPLC is not a guessing game or a series of random events, it is completely understandable. Resolution (separation) is a function of ONLY three easy to control parameters, and all students will leave knowing how to control them. Guessing what to do next will become a thing of the past! On top of all that, the learning experience at Axion is easy, fun, and takes place right in the heart of wonderful Chicago, IL!

Every participant will have the opportunity to build a calibration curve, quantify unknown samples, develop complex methods from scratch, and optimize HPLC methods to run in less than one minute! No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course, and while some experience in HPLC or introductory coursework in organic chemistry is desirable, it is far from necessary. The only prerequisite is a desire to learn HPLC. If you want to learn, we can teach you!

Plus awesome lunches are provided!


What Our Students Say


Hi I just wanted to take a minute to tell you what an amazing course Axion’s LC/GC Bootcamp is. I was fortunate enough to attend Lee’s course in the summer of 2021.  I’m a trained chromatographer with a focus in mass spectrometry.  Despite having a PhD in the field with years of study behind me, I was able to garner new information to expand my knowledge base. I highly encourage anyone to attend his course if you are afforded the opportunity.

Tiffany Liden, Ph.D.
Postdoc Researcher, Adjunct Professor  Department of Chemistry and Biochemistry, The University of Texas at Arlington
{Over the course of my career, I have attended several GC and HPLC trainings. This was the first training in which I left the course with a level of understanding that I could maximize the equipment. Rather than leaving with a tip or a trick for method development, Dr. Polite provided a complete roadmap to method development from theory to application in the training. It was enjoyable to go from the art of method development to the science of method development. For anyone looking to learn GC/HPLC, increase their lab skill sets, or make the transition from experienced user to expert; I would highly recommend this course.
Joe Block
{I'm glad I had the opportunity to attend two of the courses Axion offers. Dr. Lee Polite is one of the best teachers I ever met and his knowledge over the LC subject is amazing. I definitely recommend anyone that wants to learn about chromatography to attend the courses offered by this institute.
Klleber Oliveira
Senior Scientist, Quality Control USANA Health Sciences Inc.
{The team at Axion Labs offers a level of personalized and high-quality instruction that is nearly impossible to find anywhere else. Given how challenging of a technique chromatography is, I was blown away by the ability of every instructor to break it down into lessons that were easy to understand and retain. I came into an Axion class knowing the bare minimum about chromatography and left with the tools/confidence needed to solve various chromatography problems in the lab.  
Stephen Braunewell
Associate Scientist II bluebird bio
{...was a game changer for my career. It single handedly helped me get every job I have ever had.  I was in metrology at APP pharmaceuticals. It helped me move to AD at a startup and later a third party field service tech and now owning my own service business. I am very thankful for my experience.
Michele McClain
Owner LakeShore Scientific
{Clearly explained topic with excellent detail, gave examples of applications relevant to my job application.
Cathleen Roush
Air Pollution Specialist Air Resource Board, California
{Great Instructor and staff! I enjoyed the "hands-on" part of this course. This course really broke down GC and the fundamentals.
Carla Reguen
Forensic Chemist Wilmington Police Dept. Crime Lab
{This course was extremely helpful in teaching the fundamentals of GC. The instructor presented the material in a fashion that was easily understandable.
Monika Kothari
Lab Technician Nalco Water

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